Analysis of Extended Spectrum Beta Lactamase Frequency in Klebsiella spp Isolates

Authors : Mehmet Tevfik BAYRAKTAR, Esma CEYLAN, Bashar IBRAHIM

Pages : 1-10

Doi:10.22312/sdusbed.1206024

View : 24 | Download : 8

Publication Date : 2023-04-13

Article Type : Research Paper

Abstract :The issue of increasing resistance to antibiotics in recent years has become an important problem all over the world. Our aim is to determine the antimicrobial resistance profile and Extended Spectrum Beta-Lactamase insert ignore into journalissuearticles values(ESBL); rates in Klebsiella spp isolates to prevent the gradual increase in multi-resistant isolates as a result of unconscious antibiotic use thereby contributing to the faster effective treatment of infections. A total of 100 Klebsiella spp were isolated and identified from various clinical specimens. Antibiotic susceptibility tests were performed using the Kirby-Bauer method. The presence of extended-spectrum beta-lactamases insert ignore into journalissuearticles values(ESBL); was detected using the Double Disc Synergy Test insert ignore into journalissuearticles values(DDST); and E-test methods. The rates of ESBL-producing strains were 46.1% in 6 K. oxytoca and 56.3% in 49 K. pneumoniae. These strains were found to be 38% in 38 adult patients and 17% in 17 pediatric patients, and this difference was statistically significant insert ignore into journalissuearticles values(p <0.05);. The ESBL rate was 31% in 31 male patients and 24% in 24 female patients, and this difference was not statistically significant insert ignore into journalissuearticles values(p>0.05);. This rate was found to be high in patients hospitalized in the pediatric service and intensive care unit. 67 out of 100 strains were found to be suspicious for ESBL by Disk Diffusion Test insert ignore into journalissuearticles values(DDT);. DDST and E-tests were applied as confirmatory tests. The sensitivity of the DDST and E tests was 100%. Screening for ESBL in Klebsiella spp and other members of Enterobacteriaceae isolates is necessary to reduce further selection and spread of these increasingly broad-spectrum antimicrobial-resistant enteric pathogens.
Keywords : Klebsiella spp, Genişletilmiş Spektrumlu Beta laktamaz ESBL, Çift Disk Sinerji Testi, E Testi