Investigation of thiol/disulfide homeostasis changes and their relationship with prognosis in sepsis patients

Authors : Semiha Solak Grassie, Ş Deniz Atakent, Havva Şahin Kavakli, Filiz Banu Çetinkaya Ethemoğlu, Ahmet Rıfat Balik, Özcan Erel

Pages : 13-18

Doi:10.21673/anadoluklin.1248015

View : 35 | Download : 43

Publication Date : 2024-01-27

Article Type : Research Paper

Abstract :Aim: Sepsis is the most common life-threatening syndrome. Oxidative stress is one of the mechanisms involved in the pathogenesis of sepsis. Thiols are antioxidant buffers. Changes in thiol parameters refer to changes in oxidative balance. In this study, we aimed to investigate thiol homeostasis changes in sepsis patients. Methods: We included 99 patients (53 patients diagnosed with sepsis in the Intensive Care Unit and 46 controls). Sepsis patients were divided into two groups based on their diagnosis, follow-up, and disease severity: sepsis and septic shock. Total thiol and native thiol levels were analyzed in the patients and controls. Disulfide levels were calculated. The thiol parameters were compared between the patient and control groups, sepsis and septic shock, mortality, and survivor groups. Results: In sepsis patients, the disulfide/native thiol and disulfide/total thiol rates were significantly higher (p <0.001; <0.001), and the native thiol and total thiol levels were lower (p <0.001; <0.001) than controls. Compared to the sepsis group, the disulfide level was higher p:0.001, and native thiol and total thiol levels were lower (p <0.001; 0.009) in the septic shock patients. The results from patients who survived showed no statistical difference from those patients who died. Conclusion: The differences in thiol homeostasis parameters showed that sepsis patients had higher oxidative stress compared to the controls. The oxidative stress changes were parallel to the disease severity. The septic shock patients had higher oxidative stress compared to the sepsis patients. Diagnosis is still a problem despite the many new biomarkers for sepsis. Analyzing thiol parameters and assessing them will contribute to the diagnosis and follow-up of sepsis patients.
Keywords : Oksidatif stress, prognoz, septik şok, sepsis