The Effect of Cobalamin (Vitamin B12) Application on Some Growth, Biochemical Parameters and Pv-MATE14 Gene Expression Level in Bush Bean (Phaseolus vulgaris L.) Plants Exposed to Heavy Metal (CuSO4) Stress

Authors : Mustafa Akçay

Pages : 615-637

Doi:10.7161/omuanajas.1618560

View : 84 | Download : 267

Publication Date : 2025-10-31

Article Type : Research Paper

Abstract :Copper (Cu) toxicity poses a significant threat to plant health, particularly as Cu levels in agricultural soils rise due to the use of Cu-based agrochemicals. The role of Vitamin B12 in mitigating this heavy metal stress in common bean has not been fully elucidated. Therefore, this study investigated the effects of copper(II) sulfate (CuSO4), Vitamin B12, and their combination treatments on growth parameters, key biochemical indices, and Pv-MATE14 gene expression in bush bean (Phaseolus vulgaris L.) seedlings. CuSO4 was applied at 50 and 125 ppm, and Vitamin B12 at 50 ppm. Solutions were applied to the soil in 60 mL aliquots every two days for a total of 14 days. At the end of the treatment period, we measured plant height, root and stem lengths, fresh weights, relative water content (RWC), proline, MDA contents, and Pv-MATE14 expression. Results showed that CuSO4 treatments significantly decreased all growth parameters and RWC, while drastically increasing proline and MDA content. CuSO4 also significantly upregulated Pv-MATE14 expression, severely inhibiting growth and development. Conversely, Vitamin B12 application increased growth parameters and RWC, decreased proline and MDA content, and significantly downregulated Pv-MATE14 expression. These findings demonstrate that Vitamin B12 effectively mitigates the adverse effects of CuSO4 stress by promoting growth, alleviating cellular damage, and normalizing the expression of the Pv-MATE14 metal transporter gene in common bean.
Keywords : CuSO4, Phaseolus vulgaris L., Vitamin B12, MDA, Prolin, Pv-MATE14

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