A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions

Authors : Berkant İsmail YILDIZ, Emel TÜTEN, Sinan AYDIN, Yaprak KARADUMAN ASLAN, Ramazan ÇETİN, Erdem SUR, Kemal KARABAĞ

Pages : 271-282

Doi:10.16970/entoted.1273612

View : 64 | Download : 46

Publication Date : 2023-10-09

Article Type : Research Paper

Abstract :The Caucasian honeybee, Apis mellifera caucasica Pollmann, 1889 insert ignore into journalissuearticles values(Hymenoptera: Apidae);, is one of the most productive bee subspecies. This subspecies, which has special importance for Türkiye, has been taken under protection in two isolated regions insert ignore into journalissuearticles values(Artvin and Ardahan); since 2000. To date, no study has been conducted on whether genetic diversity has decreased in these protected Caucasian honeybee colonies. Therefore in 2022, worker bees were collected from 100 different colonies in 15 different locations in these two regions and their genetic variations were examined using 30 microsatellite loci. The average number of alleles per locus was 13.57, and the loci had a high level of information content according to the PIC insert ignore into journalissuearticles values(0.7); value. FIS insert ignore into journalissuearticles values(0.96); and FST insert ignore into journalissuearticles values(0.01); values showed low genetic diversity and high inbreeding in populations. Genetic variations were calculated as 0.77% among populations, 99.23% among individuals in populations, and 0% among all individuals. Also, populations deviated from the Hardy-Weinberg equilibrium insert ignore into journalissuearticles values(p<0.001);. Significant bottleneck evidence was found for Artvin in the analysis results using the two-phase mutation model. These results provide important information that can be used as a guide for Caucasian bee breeding strategies and conservation programs.
Keywords : Apis mellifera, kafkas arısı, genetik varyasyon, mikrosatelit, popülasyon yapısı