Increase in ANGPTL4 Levels and Its Relationship with Metabolic Parameters in Prediabetes Model

Authors : Pınar Ellergezen, Yusuf Berkcan Yanar, Gülce Sevdar Çeçen, Sinan Çavun

Pages : 387-393

Doi:10.32708/uutfd.1666630

View : 82 | Download : 189

Publication Date : 2025-12-08

Article Type : Research Paper

Abstract :Prediabetes is a condition characterized by insulin resistance, impaired glucose tolerance, and metabolic imbalance, occurring before the onset of diabetes. Angiopoietin-like protein 4 (ANGPTL4), known for its role in lipid metabolism and energy regulation, has been proposed as a potential biomarker in the progression of prediabetes due to its regulatory role in lipid metabolism and systemic metabolic homeostasis. However, data on ANGPTL4 levels in prediabetic individuals and their association with metabolic parameters remain limited. This study aimed to investigate ANGPTL4 levels in a rat model of prediabetes and assess its relationship with key metabolic indicators. Male Wistar rats (6–8 weeks old) were randomly assigned to two groups: prediabetic (n=24) and healthy controls (n=22). The prediabetic group was fed a high-fat diet for 12 weeks, while the control group received a standard diet. Blood samples were collected, and ANGPTL4 levels were measured using the ELISA method. Metabolic parameters were also evaluated, including glucose, insulin, HbA1c, lipid profile (total cholesterol, LDL, HDL, triglycerides), HOMA-IR, and HOMA-β. ANGPTL4 levels were significantly elevated in the prediabetic group compared to controls (p<0.05). A significant positive correlation was found between ANGPTL4 and HDL as well as total cholesterol, suggesting its involvement in lipid regulation. Fasting glucose, insulin, HOMA-IR, and HOMA-ß were also significantly elevated in the prediabetic group compared to controls. However, no statistically significant correlations were observed between ANGPLT4 and these insulin resistance-related markers. This divergence suggests that ANGPLT4 elevation may accompany the prediabetic state without a direct linear relationship to insulin resistance marker, possibly reflecting indirect or complex pathways. ANGPTL4 may serve as a valuable early biomarker in transitioning from prediabetes to diabetes, highlighting the need for further research on its clinical relevance and mechanistic role.
Keywords : ANGPTL4, biyobelirteç, insülin direnci, prediyabet

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