Trakya’da Doğal Yayılış Gösteren Digitalis lanata Ehrh. subsp. lanata’nın (yünlü yüksükotu) Sürgün-Ucu Kültürü ve Kardenolit İçeriğinin Belirlenmesi

Authors : Buhara YÜCESAN, İsmail Eker, Luiz Eduardo Santos Lazzarini, Noreen Aslam, Aliyu Mohammad, Jose Eduardo Brasil Pereira Pinto, Wolfgang Kreis, Ekrem Gürel

Pages : 55-62

Doi:10.24180/ijaws.390582

View : 24 | Download : 13

Publication Date : 2018-06-20

Article Type : Research Paper

Abstract :Digitalis lanata, due to the presence of cardenolides in the leaves, is widely used in drug industry for the treatments of congestive heart diesases. In this present study, natural populations of D. lanata in Thrace region were collected. Thereafter, cardiac glycoside analysis of D. lanata subsp. lanata grown in natural habitats and micropropagation from shoot tips were established as a model work for a consistent and a sustainable plant production. Of the cardenolides, Lan A insert ignore into journalissuearticles values(ranging between 24.8 and 300.4 mg 100 g -1 ); and Lan C insert ignore into journalissuearticles values(42.1 and 258 mg 100 g -1 were predominantly found in the samples as compared to lanatoside B, digoxin and digitoxin. After seed germination, shoot-tip cultivation was achieved after a subsequent shoot and root formation on MS medium. Indole-3-acetic acid insert ignore into journalissuearticles values(IAA); and indole-3-butyric acid insert ignore into journalissuearticles values(IBA); depending on their concentrations were found to be effective in shoot and root formation accordingly. Maximum two shoots from shoot tips were produced after 8 weeks of cultivation, and at the end of 12 th weeks of cultivation each shoots produced an average number of 6.4 roots in 8.1 cm length. Acclimatization was also achieved successfully with a maximum survival rate insert ignore into journalissuearticles values(95 %); for 4 weeks. This study is the first report on cardenolide profiling of Thracian D. lanata at different locations, and shoot tip cultivation of the population selected under in vitro conditions.
Keywords : Digitalis lanata, Wooly foxglove, Clonal propagation, Cardenolides, Thrace