RT-PCR Analysis of Caucasian and Mugla Honey Bees by SNP Markers of Chalkbrood Disease

Authors : Ahmet OKUMUŞ, Fatih BİLGE

Pages : 196-204

Doi:10.24180/ijaws.1185587

View : 36 | Download : 47

Publication Date : 2023-08-21

Article Type : Research Paper

Abstract :Two different honey bee subspecies’ genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 SNP primers those were used for identification of chalkbrood disease resistance genes and two SNP primers those were obtained from honey bee genom sequencing. Result of SNP analyses, four primers insert ignore into journalissuearticles values(AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140); amplified in Caucasian honey bee and six insert ignore into journalissuearticles values(AMB-00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00674355, AMB-00686140); primers amplified in Mugla honey bee. Four amplicons are similar for this subspecies. Result of electrophoresis analyses, five primers insert ignore into journalissuearticles values(AMB00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00686140); form a band in Caucasian honey bee, seven amplicons insert ignore into journalissuearticles values(AMB-00858574, AMB00612262, AMB-01151447, AMB-00631190, AMB-00902548, AMB-00674355, AMB-00686140); form a band in Mugla honey bee and four amplicons insert ignore into journalissuearticles values(AMB00858574, AMB-01151447, AMB-00631190, AMB-00686140); similar for this subspecies. As a result of this study, in Caucasian and Muğla honeybee for identification of disease resistance and evaluability as a identification key for this subspecies was emerged the capacity of association of single nucleotide polymorphisms to resistance to chalkbrood in two important honeybee genotypes in country of Apis mellifera using RT-PCR for HRM analysis.
Keywords : Bal arısı, Mugla arısı, Kafkas arısı, SNP, RT PCR, HRM, kireç hastalığı