Molecular Characterization and Identification of Micromonospora Genus Bacteria from the Van Lake Basin

Authors : Metin Ertaş, Kerem Özdemir

Pages : 932-967

Doi:10.53433/yyufbed.1739734

View : 78 | Download : 160

Publication Date : 2025-12-24

Article Type : Research Paper

Abstract :In this study, Micromonospora species were isolated from soil and sediment samples collected from the Van Lake Basin and characterized through a polyphasic taxonomic approach combining phenotypic, biochemical, and molecular (16S rRNA gene) analyses to elucidate their phylogenetic relationships and diversity. The dilution plate method was employed for isolation, yielding 141 purified Micromonospora isolates. Colonies with distinct morphologies were selected, numbered, and preserved in cryogenic tubes. The moisture and pH levels of the soil and sediment samples were also measured. Based on colony pigmentation, the isolates were classified into 11 color groups. For numerical taxonomy, 83 morphological and biochemical characteristics were assessed, and a dendrogram was constructed using the Simple Matching Coefficient (SSM) in MVSP 3.1 software. As a result, 22 clusters (7 major and 15 minor) were formed based on 78% similarity. Cluster members generally showed similarity in altitude and geographic origin. Scanning Electron Microscopy (SEM) was used to analyze the spore chain morphology, contributing to more accurate identification. For molecular characterization, genomic DNA was extracted from 13 selected isolates, and the 16S rRNA gene was amplified using universal primers 27F and 1492R. Phylogenetic trees were constructed using Maximum Likelihood and Bayesian inference algorithms. The resulting topologies were largely congruent, and Micromonospora isolates clustered with reference strains from NCBI with high sequence homology. These findings revealed the genetic positions of the isolates and provided insight into their evolutionary relationships, contributing to the microbial diversity knowledge of alkaline environments such as the Van Lake Basin.
Keywords : Micromonospora, Moleküler karakterizasyon, Van Gölü Havzası, 16S rRNA

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