Callus Production in Geranium (Pelargonium quercetorum Agnew) Growing Naturally in Türkiye

Authors : Metin KOÇAK, Sibel TURAN SİRKE, Cansu KUZĞUN, Mehtap YILDIZ

Pages : 503-512

Doi:10.29133/yyutbd.1307775

View : 72 | Download : 119

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :Pelargonium quercetorum Agnew grows naturally in the Hakkari province of Türkiye. Although P. quercetorum Agnew has potential use as a medicine and ornamental plant, it is especially used as a medicinal plant for the cure of various diseases by local people. In vitro tissue culture methods are favorable for the propagation, conservation, and breeding of medicinal plants. We aimed in this study to achieve regeneration of P. quercetorum Agnew from different explant types. Seeds of P. quercetorum Agnew were germinated in vitro conditions and explants were taken from these germinated sterile plantlets. Totally four different experiments, containing three of them embryogenic and one of them organogenic culture, were established to achieve regeneration in P. quercetorum Agnew. Leaf, petiole, cotyledon, cotyledon stalk, and root collar disc were used as explant. Different concentrations of 1-Naphthaleneacetic acid insert ignore into journalissuearticles values(NAA);, 2,4-dichlorophenoxyacetic insert ignore into journalissuearticles values(2,4-D);, 6-Benzylaminopurine insert ignore into journalissuearticles values(BA);, 6-Furfurylaminopurine insert ignore into journalissuearticles values(Kinetin);, 6-insert ignore into journalissuearticles values(γ,γ-Dimethylallylamino); purine insert ignore into journalissuearticles values(2iP);, and Thidiazuron insert ignore into journalissuearticles values(TDZ); were used to induce embryogenic or organogenic regeneration. Explants were cultured in half-strength or full-strength Murashige and Skoog insert ignore into journalissuearticles values(MS); medium. In the embryogenic experiments, callus formation from different media ranged from 63.5% to 100%, and for explant types ranged from 39% to 100%. In the organogenic experiment, callus formation from different media ranged from 12.5% to 100%, and for explant types ranged from 71% to 93%. Also, embryo-like structures were obtained from embryogenic experiments. However, these structures could not grow more and transformed into plantlets.
Keywords : Pelargonium quercetorum Agnew, Somatic embryogenesis, Organogenesis, Callus