DEVELOPMENT AND CHARACTERIZATION OF ASCORBIC ACID LOADED POLYELECTROLYTE CHITOSAN-GELATIN HYDROGELS

Authors : Tuğba EREN BÖNCÜ, Çiğdem YÜCEL

Pages : 719-730

Doi:10.33483/jfpau.1269790

View : 182 | Download : 171

Publication Date : 2023-09-20

Article Type : Research Paper

Abstract :Objective: Aim of study was to formulate chitosan-gelatin hydrogels containing ascorbic acid, an antioxidant, with/without polyelectrolyte-complex. Material and Method: Effect of formation polyelectrolyte-complex, gelatin concentration insert ignore into journalissuearticles values(10-20%); and chitosan:gelatin ratioinsert ignore into journalissuearticles values(1:1, 1:2, 2:1w/w); on the rheological properties, in-vitro release, encapsulation efficiency of hydrogels were investigated. Dissolution rates were also compared using area under dissolution curve insert ignore into journalissuearticles values(AUC);, mean dissolution time insert ignore into journalissuearticles values(MDT);, mean residence time insert ignore into journalissuearticles values(MRT);. Also, the potential for topical use of the hydrogel was evaluated by examining the 24-and 72-hours cytotoxic and proliferative effects on L929 cell line using MTT test. Result and Discussion: Polyelectrolyte complex formation led to improved drug release and increased viscosity. Cell viability of the free and drug-loaded polyelectrolyte-hydrogels was over 70% at the end of the 72h in all formulations insert ignore into journalissuearticles values(except formulations with chitosan:gelatin ratio of 1:2w/w); showed that ascorbic acid and hydrogels did not cause cellular toxicity and could be used safely. It has been demonstrated that the gelatin ratio should be at most 50%, and excess gelatin reduces cell viability. F6-coded-polyelectrolyte-hydrogel insert ignore into journalissuearticles values(20% gelatin; 2:1 chitosan:gelatin w/w); was ideal formulation as it led to best sustained drug release with high MDT and AUC values, and cell viability >80%. In conclusion, polyelectrolyte-complex formation is more superior, and chitosan:gelatin ratio and gelatin concentration can be manipulated to obtain the desired properties.
Keywords : Askorbik asit vitamin C, hidrojeller, jelatin, kitozan, polielektrolit kompleks kitozan jelatin