TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Authors : Laid Ziane, Abdelaziz Berreghioua

Pages : 1313-1317

Doi:10.33483/jfpau.1461426

View : 27 | Download : 53

Publication Date : 2024-11-25

Article Type : Research Paper

Abstract :Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents. Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plant\'s antioxidant activity, were carried out using the conventional procedures. Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent (GAE)/g dry extract) and 439 mg quercetin equivalent (QE)/g dry extract. The hydro-alcoholic extract was extracted by liquid/liquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol) by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mg/ml, respectively.
Keywords : Antioksidan aktivite, Atriplex halimus, biyoaktif ekstre, DPPH, fitokimyasal, quercetin