ZERO-ORDER AND FIRST-DERIVATIVE SPECTROPHOTOMETRY FOR THE DETERMINATION OF SPIRONOLACTONE IN PHARMACEUTICAL TABLETS

Authors : Asiye Üçer, Nezaket Başaran, Erdal Dinç, Özgür Üstündağ

Pages : 672-680

Doi:10.33483/jfpau.1608315

View : 57 | Download : 99

Publication Date : 2025-09-19

Article Type : Research Paper

Abstract :Objective: Spironolactone, a potassium-sparing diuretic drug on the WHO\\\'s Essential Medicines List, is primarily used to treat fluid retention resulting from heart failure, kidney disease, and liver scarring. In this research article, zero-order, and first-derivative spectrophotometric approaches were applied to the quantity of spironolactone in pharmaceutical tablet formulation without any separation procedure. Material and Method: Spironolactone compound was obtained from Sigma-Aldrich (Steinheim, Germany). As a solvent methanol (J.T. Baker, Netherlands, HPLC grade) was used for UV-visible spectrophotometric assays. In the implementation of the zero-order and first-derivative approaches, the quantification of Spironolactone was carried out using UV spectrophotometric measurements in the 200-300 nm wavelength range (with a 2 nm increment) and 1 cm quartz cells. These approaches were tested by analyzing spironolactone in independent test samples and spiked samples. The tablet analyses of spironolactone were performed on ALDACTONE®-A 25 mg Tablet supplied by Aris Ali Raif Pharmaceuticals Industry, Başakşehir, İstanbul, Türkiye. Result and Discussion: The analysis of spironolactone was conducted using zero-order values measured at 239 nm and first-derivative values measured at 250.4 nm (n=3). The calibration graphs created at these wavelengths showed linearity within the 6.0 to 20.0 µg/ml concentration range, with correlation coefficients of r = 0.9996 for the zero-order method and r = 0.9997 for the first-order derivative method. The validity of the methods was proved by analyzing spironolactone in independent test samples and spiked samples. These methods were then applied to the quantitation of spironolactone in commercial tablets and a good agreement was reported between label claim assay results.
Keywords : Antihipertansif ilaç, birinci türev spektrofotometri, sıfırıncı mertebe spektrofotometri, spironolakton tayini, tablet analizi

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