The Effects of Biotics Derived from Lactiplantibacillus plantarum EIR/IF-1 on the Modulation of Periodontal Inflammation

Authors : Hikmet Can, Hazal Kibar Demirhan, Emine Omer Oglou, Fadime Kıran

Pages : 1-15

View : 39 | Download : 37

Publication Date : 2025-04-30

Article Type : Research Paper

Abstract :Objective: This study aims to investigate the immunomodulatory effects of postbiotics and paraprobiotics derived from Lactiplantibacillus plantarum EIR/IF-1, isolated from infant fecal microbiota. Materials and Methods: Human periodontal ligament fibroblast (hPDLF) cells were co-incubated with the release and bound forms of exopolysaccharides (10-1,000 μg/mL), cell lysate (0.1-1,000 μg/mL), cell surface proteins (0.1-100 μg/mL), and inactivated cells (10⁶-10¹⁰ CFU/mL) derived from Lactiplantibacillus plantarum EIR/IF-1 for 24 hours. Cell viability was assessed using the MTT assay. Lipopolysaccharide (LPS) from Porphyromonas gingivalis, a key pathogen involved in periodontal disease, was used to induce inflammation in hPDLF cells. Non-toxic doses of the biotics were subsequently tested for their impact on cytokine production in LPS induced hPDLF cells. Cytokine levels (IL-10, IL-8, and IFN-γ) were quantified using the ELISA protocol. Results: After 24 hours incubation of hPDLF cells with 1 µg/mL LPS and selected concentrations of the biotics derived from the EIR/IF-1 strain that did not exhibit toxic effects, it was observed that LPS from P. gingivalis induced IL-8 production. However, the biotics extracted from the EIR/IF-1 strain significantly reduced IL-8 production (p<0.0001). Furthermore, these biotics increased the production of the anti-inflammatory cytokine IL-10 (p<0.0001). Conclusion: The obtained data suggest that the biotics have the potential to counteract the pro-inflammatory effects induced by LPS. The findings highlight the potential of postbiotics and paraprobiotics, containing biologically active and effective components, as a promising natural and reliable approach for reducing and preventing periodontal inflammation. In conclusion, our results suggest that microbiota-derived biotics could serve as effective adjuncts in the management of periodontal diseases.
Keywords : İnflamasyon, Lactiplantibacillus plantarum, Paraprobiyotikler, Postbiyotikler