Antifungal Efficacy of Mint Essential Oil Against Penicillium spp. Inoculated on Carrots

Authors : Veronika VALKOVÁ, Hana ĎÚRANOVÁ, Lucia GALOVİČOVÁ, Nenad VUKOVİC, Milena VUKİC, Miroslava KAČÁNİOVÁ

Pages : 9-16

Doi:10.30516/bilgesci.1206873

View : 26 | Download : 7

Publication Date : 2023-03-31

Article Type : Research Paper

Abstract :Current research aims to investigate the chemical composition, antioxidant, and in vitro and in situ antifungal activities of commercially available Mentha arvensis essential oil insert ignore into journalissuearticles values(mint, MEO);. The identification of the volatile substances was done using Gas chromatography–Mass spectrometry insert ignore into journalissuearticles values(GC-MS); analysis. A total of 42 components representing 99.5% of the total oil were identified. The main compounds in the oil were menthol insert ignore into journalissuearticles values(37.3%);, menthone insert ignore into journalissuearticles values(17.4%);, neo-menthol insert ignore into journalissuearticles values(14.1%);, and 1,8-cineole insert ignore into journalissuearticles values(4.9%);. Antioxidant assays insert ignore into journalissuearticles values(1,1-diphenyl-2-picrylhydrazyl radical); demonstrate only weak activity for the MEO in values 195.00 ± 5.30 μg TEAC.mL-1, with 22.8 ± 1.2% free radical-scavenging inhibition. Evaluation of in vitro and in situ antifungal activities of MEO insert ignore into journalissuearticles values(in four concentrations: 62.5 µL/L, 125 µL/L, 250 µL/L, and 250 µL/L); against three strains of Penicillium insert ignore into journalissuearticles values(P.); spp. fungi strains insert ignore into journalissuearticles values(P. expansum, P. citrinum, P. crustosum); were assessed by disc diffusion method and vapor contact method on the carrot as model food, respectively. The suitability of carrots as a substrate for analyzes was verified by determining moisture content insert ignore into journalissuearticles values(MC); and water activity insert ignore into journalissuearticles values(aw);, which showed values of 82.80 ± 2.33% and 0.959 ± 0.001, respectively. MEO exhibited promising antifungal activity against analyzed strains of test fungi as a diameter of zones of inhibition insert ignore into journalissuearticles values(from 2.88 ± 0.55 to 12.33 ± 1.14 mm);, as well as the effectiveness of this oil was detected on the carrot model insert ignore into journalissuearticles values(from -5.41 ± 7.35 to 100.00 ± 0.00%);. Moreover, it can be concluded that the growth inhibition of fungi strains significantly depends insert ignore into journalissuearticles values(P < 0.05); on the concentration of the MEO used in both procedures. Our results suggest that MEO, as a promising natural antifungal agent, can be applied in the innovative packaging of food products including carrots.
Keywords : Mentha arvensis, DPPH assay, volatile compounds, antifungal activity, model food