Purification and Characterization of Glutathione Reductase Enzyme from Arum Maculatum Leaf

Authors : Gürkan BİLİR, Mücella SARIAHMET, Deniz EKİNCİ

Pages : 269-274

Doi:10.47115/bsagriculture.1247272

View : 15 | Download : 10

Publication Date : 2023-05-01

Article Type : Research Paper

Abstract :Arum species grow in temperate and Mediterranean climates and have been used for hundreds of years for food and medicinal purposes, although they are highly toxic if not cooked using proper techniques. Glutathione reductase insert ignore into journalissuearticles values(GR); is a member of the pyridine nucleotide disulfide oxidoreductase family of flavoenzymes that catalyzes the reduction of glutathione disulfide insert ignore into journalissuearticles values(GSSG); to reduced GSH using NADPH or NADH. In this study, GR enzyme was characterized by partial purification processes including homogenate preparation, ammonium sulfate precipitation and dialysis from the leaf of Arum maculatum plant. The highest enzyme activity was found at 40-60% saturation range. Optimum ionic strength, pH and substrate concentration were investigated for GR enzyme from A. maculatum leaf. As a result of the study, these values were found to be 150 mM potassium phosphate buffer, pH 7.00, and 0.18 mM, respectively. The GR enzyme was partially purified from the leaf of the A. maculatum with a specific activity of 1.640 EU mg-1 in 34.9% yield, 1.108-fold. This study is the first study in terms of purification and characterization of GR enzyme from A. maculatum leaf.
Keywords : Glutathione reductase, Arum maculatum, Purification, Enzyme, Characterization