Evaluation of Neutral Electrolyzed Water as a Potential Fig Processing Surfaces Sanitizer in the Fig Industry

Authors : Çiğdem YAMANER, Ramazan KONAK

Pages : 254-261

Doi:10.47115/bsagriculture.1247353

View : 27 | Download : 13

Publication Date : 2023-05-01

Article Type : Research Paper

Abstract :In this study, the antimicrobial activity of neutral electrolyzed water insert ignore into journalissuearticles values(NEW); on Bacillus cereus forming endospore, Escherichia coli and, toxin producer Aspergillus flavus and Penicillium expansum was determined both on the surface of steel plates in the presence of organic matter artificially inoculated and in cell suspensions. Also, the antimicrobial efficiency of NEW was compared to that of Sodium hypochlorite insert ignore into journalissuearticles values(NaClO);. Experiments were carried out at room temperature insert ignore into journalissuearticles values(22 °C);. 1% sodium hypochlorite solution insert ignore into journalissuearticles values(with 531 ppm free chlorine);, and different concentrations of NEW, 5% insert ignore into journalissuearticles values(with 63 ppm free chlorine);, 10% insert ignore into journalissuearticles values(with 120 ppm free chlorine);, and 15% insert ignore into journalissuearticles values(with 187 ppm free chlorine); were used for the comparison. Cell suspensions and stainless-steel plates inoculated with a final 10% liquid fig solution were treated with NEW and NaClO for 0 insert ignore into journalissuearticles values(untreated, control);, 15, 30, and 60 seconds. Then, viable cell counts both in cell suspensions and on the inoculated stainless-steel plates were determined. It was determined that there were significant differences insert ignore into journalissuearticles values(P<0.05); in the decrease in the number of microorganisms depending on the application time and free chlorine concentration. The reduction ratios insert ignore into journalissuearticles values(%); in cell suspensions after 60 seconds of treatment with NEW ranged from 48.8 to 100 for E. coli, 11.39 – 32.23 for B. cereus and, 31.12 – 100 for A. flavus. The reduction ratio for P. expansum was %100 for all concentrations of NEW after 60 sec. After 60 seconds application of 1% NaClO to the cell suspensions, the reduction ratios insert ignore into journalissuearticles values(%); were determined to be 29.56, 23.48, 39.19 and 69.92 for E. coli, B. cereus, A. flavus and P. expansum, respectively. However, in the experiments performed after inoculation of microorganisms and sterile 10% liquid fig solution on the surface of steel plates, it was observed that microorganisms showed greater resistance to NEW and 1% NaClO compared to direct application to the cell suspension. The reduction ratios insert ignore into journalissuearticles values(%); on the surface of steel plates after 60 seconds of treatment with NEW ranged from 17.66 to 40.07 for E. coli, 23.93–31.77 for B. cereus, 10,91–30,91 for A. flavus and, 49.77–64.85 for P. expansum. After 60 seconds application of 1% NaClO on the surface of steel plates, the reduction ratios insert ignore into journalissuearticles values(%); were 19.38, 11.70, 7.5 and 46.52 for E. coli, B. cereus, A. flavus and P. expansum, respectively. The results of this study showed that 15% NEW can be used as a strong bactericide and fungicide against endospore-forming bacteria and toxin-producing fungi. Also, 15% NEW is more effective than 1% NaClO in cleaning the surfaces used for fig processing. Therefore, NEW also can be a good alternative to commonly used disinfectants. This is the first report on the use of NEW as a fungicide and bactericide on fig processing surfaces in the fig industry.
Keywords : Neutral electrolyzed water, fig industry, endospore forming bacteria, E coli, Aspergillus flavus, Penicillium expansum