Cytotoxic activity of TRPV4 antagonist RN-1734 in G-361 human melanoma cancer cell line

Authors : Özay GÜLEŞ, Esra BİLİCİ, Emira KURBASEVIC, Ömer Faruk LENGER, Murat BOYACIOĞLU, Erkmen Tuğrul EPİKMEN

Pages : 939-947

Doi:10.17826/cumj.1324675

View : 48 | Download : 65

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :Purpose: Intracellular calcium insert ignore into journalissuearticles values(Ca2+); signaling plays a role in many cellular events, such as cell proliferation and differentiation, gene transcription, oxidative stress, the antioxidant system, and apoptosis. Transient receptor potential vanilloid 4 insert ignore into journalissuearticles values(TRPV4); channels are non-selective cation insert ignore into journalissuearticles values(Ca2+); channels. The present study aims to investigate the cytotoxic activity of RN-1734, a transient receptor potential vanilloid 4 insert ignore into journalissuearticles values(TRPV4); antagonist, in the G361 human melanoma cancer cell line. Materials and Methods: The effects of RN-1734 on G361 cell viability at concentrations of 1, 5, 25, 50, and 100 μM were measured using the 3-insert ignore into journalissuearticles values(4,5-dimethylthiazol-2-il);-2,5-diphenyltetrazolium bromide insert ignore into journalissuearticles values(MTT); method. Total antioxidant status insert ignore into journalissuearticles values(TAS); and total oxidant status insert ignore into journalissuearticles values(TOS); levels were determined using a ready-made commercial kit, after which oxidative stress index insert ignore into journalissuearticles values(OSI); values were calculated. To determine the apoptotic effects of RN-1734, Bcl-2, Bax, and p53 expression levels, caspase-3 and -8 activities were examined via quantitative real-time PCR analysis. Results: G361 cell viability significantly decreased to 82.72, 72.81, 56.36, 39.16 and 18.96% in RN-1734 groups insert ignore into journalissuearticles values(1, 5, 25, 50 and 100 μM); compared to the control group insert ignore into journalissuearticles values(100.00%);. At IC50 concentration insert ignore into journalissuearticles values(39.48 μM);, RN-1734 application insert ignore into journalissuearticles values(3.35 mmol/g prot.-TAS, 45.87 μmol/g prot.-TOS, and 1501.97 AU-OSI); increased the TAS level insert ignore into journalissuearticles values(2.17 mmol/g prot.); and decreased the TOS level insert ignore into journalissuearticles values(55.41 μmol/g prot.); and OSI value insert ignore into journalissuearticles values(3142.76 AU); compared to the control group. Conclusion: Our findings show that RN-1734 may be a novel therapeutic approach to treating melanoma by decreasing the cell viability of G361 human melanoma cancer cells.
Keywords : Hücre canlılığı, G361 insan melanom kanser hücreleri, oksidatif stres, RN 1734, TRPV4