Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells

Authors : Bahadır Batar, Mehmet Kazancı, Ahmet Ensar Karaoğlu, Alperen Genç, Furkan Doğan, Zeynel Utku Reyhan, Dilara Ceren Çaba, Yasemin Günaydın, Muhammed Baran, Eslem Mortaş, Ayşe Nur Çakır, Ferhat Eyüp Yeşilmen, Ada Aslan, Öykü Canbulat, Büşra Berdibek, Elif Serdal, Merve Argon, Hilmican Çalışkan, Temine Şabudak, Birol Topçu

Pages : 752-760

Doi:10.17826/cumj.1669161

View : 25 | Download : 57

Publication Date : 2025-09-30

Article Type : Research Paper

Abstract :Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum (Savi) DC. plants in breast cancer cells. Materials and Methods: MDA-MB-231 triple-negative breast cancer (TNBC) cells were used to demonstrate reactive oxygen species (ROS) production effects and histone deacetylase (HDAC) inhibitory activity of flavonoids [5,3\\\',4\\\'-trihydroxy-7-methoxy isoflavone (Santal) (1), 5,5\\\'\\\',7\\\'\\\'-trihydroxy-3,7-dimethoxy-4\\\',4\\\'\\\'\\\'-O-biflavone (2), 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside (3), (8,3\\\',4\\\',5\\\'-tetrahydroxy-7-O-α-glycosyl isoflavone)-6-8\\\'\\\'-(4\\\'\\\'\\\'-hydroxy-7\\\'\\\'-O-α-glycosyl isoflavone) biflavone (4)] isolated from C. italicum. Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mg/ml. Compound 2 at 0.06 mg/ml, and compounds 2 and 4 at 0.08 and 0.1 mg/ml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mg/ml, compound 2 showed a mean value of 0.003±0.004. At 0.08 mg/ml, the mean values were 0.006±0.018 for compound 2 and 0.013±0.013 for compound 4. At 0.1 mg/ml, compound 2 and compound 4 exhibited mean values of 0.014±0.017 and 0.014±0.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant. Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.
Keywords : Asteraceae, meme kanseri, Cirsium italicum, flavonoid, histon deasetilaz, reaktif oksijen türleri

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