The osteogenic potential of cerebrospinal fluid obtained after traumatic brain injury in a rat fracture model

Authors : Alaaddin Levent Özgözen, Nazlı Soygun, İsmet Tan

Pages : 1013-1024

Doi:10.17826/cumj.1670756

View : 66 | Download : 225

Publication Date : 2025-12-22

Article Type : Research Paper

Abstract :Purpose: Accelerated fracture healing following traumatic brain injury was reported in the literature, yet the underlying mechanisms remain unclear. This study hypothesizes that cerebrospinal fluid entering systemic circulation after traumatic brain injury contributes to the acceleration of fracture healing. To test this hypothesis, we aimed to evaluate the effects of cerebrospinal fluid collected post-traumatic brain injury on fracture healing in a rat model. Materials and Methods: A total of 39 male Wistar albino rats were divided into three groups: control group (n=15), experimental group (n=14), and head trauma group (n=10). Head trauma was induced in the head trauma group, and cerebrospinal fluid was collected from the 10 rats following trauma. Femur fractures were induced and intramedullary fixation was performed in the control and experimental groups. Cerebrospinal fluid from the head trauma group was injected into the fracture site of the experimental group. On the 30th day, femurs were disarticulated, and callus tissue was evaluated radiologically for length and width and histologically using the Huo grading system. Results: In the experimental group, the mean callus tissue length was 11.96 ± 3.29 mm, whereas in the control group it was 6.96 ± 3.30 mm. The callus tissue length in the experimental group was significantly higher compared to the control group. Callus tissue thickness was measured as 1.51 ± 0.60 mm in the experimental group and 1.17 ± 0.58 mm in the control group, with no statistically significant difference between the groups. The callus maturation score in the experimental group (6.71 ± 0.73) was significantly higher compared to the control group (5.67 ± 1.05). Conclusion: The mechanism underlying accelerated fracture healing after traumatic brain injury remains elusive. In this study, cerebrospinal fluid collected post-traumatic brain injury demonstrated histologically significant acceleration of fracture healing and increased callus length.
Keywords : beyin omurilik sıvısı, kırık iyileşmesi, travmatik beyin hasarı

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