Investigation of extraction method effect on yeast beta glucan production

Authors : Fatma KARSLIOĞLU, Suna ERTUNÇ, Zeynep YİLMAZER HİTİT, Bülent AKAY

Pages : 51-55

Doi:10.46239/ejbcs.734046

View : 29 | Download : 10

Publication Date : 2021-12-26

Article Type : Research Paper

Abstract :Nowadays, due to the stressful, tiring and busy lives of humans, the immune system becomes weak and can get sick easily. Therefore, scientists have been doing researches about new, natural and healthy products that can strengthen their immune system and can provide to adapt life standards. One of these products is the beta glucan. It is a polysaccharide molecule that consists of D-glucose monomers bonding with beta glycosidic bonds. Beta insert ignore into journalissuearticles values(β); glucans have being produced from different sources insert ignore into journalissuearticles values(microorganisms, cereals and mushrooms); so they have different branched structures such as insert ignore into journalissuearticles values(1→3); insert ignore into journalissuearticles values(1→6);, insert ignore into journalissuearticles values(1→3); insert ignore into journalissuearticles values(1→4);, insert ignore into journalissuearticles values(1→3); insert ignore into journalissuearticles values(1→2);. Thus, different branched beta glucans show different physicochemical properties and biological activities that designate their usage purposes. Especially yeast beta glucan has lots of biological activities. On the other hand, it is fact that the extraction method affects the molecular weight, yield, purity and other properties of beta glucans. The main purpose of this study is to compare the performances of ultrasonically assisted alkali-acidic and autolysis extraction methods to produce a high yield of beta glucan. Also, it was aimed to determine the effect of extraction method on the molecular weight of yeast beta glucan. As a result of this work, it was found that the yeast beta glucan yield for ultrasonic supported autolysis extraction insert ignore into journalissuearticles values(12 %); is higher than that of the ultrasonic supported alkali-acidic extraction insert ignore into journalissuearticles values(8 %);. On the other hand, having the lower molecular weight of yeast beta glucan insert ignore into journalissuearticles values(87 kDa); was produced by using an ultrasonic supported autolysis extraction method.
Keywords : Beta glucan, Saccharomyces cerevisiae, yeast, alkali acidic extraction, autolysis, molecular weight