Development of a Liquid Chromatographic Method for Apomorphine Hydrochloride Quantitation in the Active Pharmaceutical Ingredients and in the Dosage Forms

Authors : Beyza Sultan AYDIN, İbrahim BULDUK

Pages : 44-50

Doi:10.46239/ejbcs.883492

View : 18 | Download : 7

Publication Date : 2021-12-26

Article Type : Research Paper

Abstract :Apomorphine is currently used as a dopamine agonist to treat advanced Parkinson Disease. In the present study, we developed a sensitive, simple, reliable, and robust HPLC method for quality control of apomorphine hydrochloride in bulk drug and pharmaceutical formulations. C18 column was used for separation. Isocratic elution was performed using mobile phase A: 50 mM potassium dihydrogen phosphate solution insert ignore into journalissuearticles values(pH:3 with ortho-phosphoric acid);, B: acetonitrile in the ratio of 85:15 insert ignore into journalissuearticles values(A: B);, and the mobile-phase flow rate was kept at 1 mL min-1. Analyses were carried out at 272 nm using a UV detector. A perfect linear relationship between peak-area versus drug concentration in the range of 10‑100 μg mL-1 was observed insert ignore into journalissuearticles values(r2, 0.9999);. It has been found that the developed method is sensitive insert ignore into journalissuearticles values(Detection and quantification limits were determined as 1.3 μg mL-1 and 3.8 μg mL-1, respectively);, precise insert ignore into journalissuearticles values(RSD <0.9%, for repeatability and <1.2% for intermediate precision, inside appropriate precision ranges);, accurate insert ignore into journalissuearticles values(recovery in various dosage forms, 99.68‑100.56%, in a reasonable range, 80‑120%);, specific and robust insert ignore into journalissuearticles values(% RSD <0.80, for system suitability parameters);. The proposed method for the determination of apomorphine hydrochloride in pharmaceutical formulations has been successfully applied.
Keywords : Apomorphine, HPLC, Pharmaceuticals, Quality control