Thymoquinone Prevents Valproic Acid-Induced Nephrotoxicity in Rat Kidney

Authors : Sebile AZİRAK, Deniz TAŞTEMİR KORKMAZ, Sedat BİLGİÇ, Meltem ÖZGÖÇMEN, Mehmet Kaya ÖZER
Pages : 77-84
Doi:10.46239/ejbcs.1123892
View : 43 | Download : 16
Publication Date : 2022-12-28
Article Type : Research Paper
Abstract :Valproic acid insert ignore into journalissuearticles values(VA);, widely used as an antiepileptic, causes structural and functional kidney disorders. Whether thymoquinone insert ignore into journalissuearticles values(TQ); has a beneficial effect on valproic acid insert ignore into journalissuearticles values(VA);-induced nephrotoxicity has been investigated. Twenty-one male Spraque Dawley rats were grouped into control, VA, and VA + TQ groups insert ignore into journalissuearticles values(n=7 for per group);. VA insert ignore into journalissuearticles values(500 mg/kg/day); and TQ insert ignore into journalissuearticles values(50 mg/kg/day); were applied to the rats orally for 14 days. They were euthanized on the 15th day of the treatment. The cyclooxygenase 1 insert ignore into journalissuearticles values(COX-1); and cyclooxygenase 2 insert ignore into journalissuearticles values(COX-2); gene expression levels, biochemical parameters, total antioxidant/oxidant statuses insert ignore into journalissuearticles values(TAS/TOS);, oxidative stress index insert ignore into journalissuearticles values(OSI);, histological and immunohistochemical analysis were performed to evaluate kidney toxicity. In the VA + TQ group, COX-1 expression levels increased, while COX-2 expression levels decreased. While the creatinine insert ignore into journalissuearticles values(Cr); and blood urea nitrogen insert ignore into journalissuearticles values(BUN); levels, production of caspase-3 insert ignore into journalissuearticles values(CAS-3); and NADPH oxidase-4 insert ignore into journalissuearticles values(NOX-4); were increased in the VA-treated group, they were decreased in VA + TQ group. Treatment with TQ against VA administration decreased TOS and OSI levels while increasing TAS. TQ protects the kidney against the toxic effects of VA.
Keywords : Apoptosis, COX 1, COX 2, Oxidative stress, Thymoquinone, Valproic acid

ORIGINAL ARTICLE URL