Tamoxifen Delivery to Breast Cancer Cells (MCF-7) Via Hydroxyapatite Microspheres

Authors : Binnaz KIRBIYIK, Birgül MAZMANCI, Şeyma Gülnaz YARLILAR, Naz UĞUR, Kasım OCAKOĞLU

Pages : 111-118

Doi:10.46239/ejbcs.1040161

View : 21 | Download : 13

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Drug delivery systems have been used in cancer treatment to increase drug effectiveness. The hydroxyapatite insert ignore into journalissuearticles values(HAP); based materials used in this area can provide drug transport to the target site without its deterioration. In this study, porous hollow hydroxyapatite microspheres insert ignore into journalissuearticles values(PHHMs); were produced by using the hydrothermal method. Tamoxifen insert ignore into journalissuearticles values(TAM); used in the treatment of breast cancer has been covalently attached to the produced microspheres. The obtained microsphere structures insert ignore into journalissuearticles values(tamoxifen-loaded hydroxyapatite, TAM/H); were successfully characterized by ATR-FTIR, FE-SEM, XRD, and DLS methods. The breast cancer cell line MCF-7 was used to examine the effect of the hybrid structure. The cytotoxic and genotoxic effects of TAM/H was compared with the TAM groups on MCF-7. Our results have showed that, the decrease in cell viability at 24 and 36 hours were still continued at 48 hours only in TAM/H groups. In addition, TAM/H was found to show a genotoxic affect by the increment in genetic damage index insert ignore into journalissuearticles values(GDI); and damaged cell percentage insert ignore into journalissuearticles values(DCP%);. As a result, use of hydroxyapatite was suitable for the transport of TAM and that covalent binding was suitable for drug particle interaction with hybrid structure and thus controlled drug release occurred.
Keywords : Hydroxyapatite microsphere, Tamoxifen, Hydrothermal method, MCF 7, Cytotoxicity, Genotoxicity