Major Phospholipids of Selected Dairy Products as Determined by the HPLC-UVvis and 31P-NMR Methods

Authors : Lemuel DİAMANTE

Pages : 127-134

Doi:10.46239/ejbcs.1141216

View : 24 | Download : 122

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :This study was carried out to determine the major phospholipids in selected dairy products insert ignore into journalissuearticles values(Beta Serum, Procream and Phospholipids-Rich Dairy products);, evaluate the accuracy of the developed fat extraction method for liquid dairy samples and to compare the major phospholipids of different dairy samples obtained using the HPLC-UVvis and 31P-NMR methods. It was found that the developed fat extraction method can be used to estimate the lipid content of liquid dairy samples were still a bit satisfactory. Using the HPLC-UVvis method, it was found that the sphingomyelin insert ignore into journalissuearticles values(SM); consists of 2 curves in Beta Serum, Procream and Phospholipid-Rich products. The phosphatidylethanolamine insert ignore into journalissuearticles values(PE); separated ahead of phosphatidylcholine insert ignore into journalissuearticles values(PC); and then PC ahead of SM1 and SM2. The results showed that the data of the major phospholipids insert ignore into journalissuearticles values(PC, PE and SM); in Butter Serum, Procream and Phospholipids-Rich products as determined by the HPLC-UVvis method compared well with those of the 31P-NMR method It must be noted that the analysis temperature for the HPLC-UVvis method was at 40oC while the 31P-NMR method was at 30oC, and this might have also contributed to the slight variation of the results. Furthermore, the HPLC-UVvis method is rapid and with cheaper analysis cost compared with the 31P-NMR method.
Keywords : Phospholipids, Phosphatidylcholine, Phosphatidylethanolamine, Sphingomyelin, HPLC UVvis, 31P NMR