Optimization of Extraction Parameters and Effect of Different Solvent Systems on The Omega-3 Fatty Acids Content of Algal Oil (Nannochloropsis sp.)

Authors : Tuğçe AYGÜN, Osman TOPUZ

Pages : 174-189

View : 23 | Download : 9

Publication Date : 2021-12-31

Article Type : Research Paper

Abstract :Ultrasonically-assisted algal oil insert ignore into journalissuearticles values(Nannocholoropsis sp.); extraction insert ignore into journalissuearticles values(UAE); was optimized using Response Surface Methodology insert ignore into journalissuearticles values(RSM); and hexane. Extraction variables were determined as extraction time, temperature, and solvent:biomass ratio. Optimization was made by aiming both maximum oil yield and omega-3 fatty acid insert ignore into journalissuearticles values(ω-3 FA); content at the same time. The optimum conditions were determined to be 44.30 ℃, 62.46 min, 19.9:1 g/ml. The extraction time and temperature significantly affected the yield and ω-3 FA content, whereas solvent: biomass ratio did not affect the range of values tested for each of the variablesinsert ignore into journalissuearticles values(p<0.05);. Then, under these optimum conditions, UAE was applied using selected solvents of different polarities insert ignore into journalissuearticles values(hexane, chloroform, methanol, ethanol and 2-propanol);. The effects of different solvents on the oil yield, ω-3 FA content, oxidation, and nutritional properties of algal oil were investigated. Methanol was found to be more efficient than other solvents considering oil yield and ω-3 FA, especially eicosapentaenoic acid insert ignore into journalissuearticles values(EPA); content insert ignore into journalissuearticles values(14.46%);. Although methanol and chloroform are widely used in extraction, their toxicity limits their use in the food industry. Considering the oil yield and ω-3 FA content of non-toxic solvents, it was determined that 2-propanol was more preferable due to its high ω-3 FA content.
Keywords : Edible algal oil, Ultrasonic assisted extraction, RSM optimization, Solvent polarity, Omega 3 fatty acids