Antioxidant and Cytotoxic Activity Studies in Series of Higher Amino Acid Schiff Bases

Authors : Özlem ÖZDEMİR, Perihan GÜRKAN, Yaprak Dilber ŞİMAY DEMİR, Mustafa ARK

Pages : 646-660

Doi:10.35378/gujs.654598

View : 22 | Download : 14

Publication Date : 2020-09-01

Article Type : Research Paper

Abstract :In this work, the antioxidant activity of the higher amino acid Schiff bases, which were prepared as the monosodium salts insert ignore into journalissuearticles values(1a–3a); and the neutral forms insert ignore into journalissuearticles values(1b–3b); was determined by DPPH scavenging assay. In pure MeOH solution, the scavenging ability of Schiff bases 1a-3a were higher than 1b-3b, but lower than ascorbic acid. The activity followed the order 3 insert ignore into journalissuearticles values(a,b); > 2 insert ignore into journalissuearticles values(a,b); > 1 insert ignore into journalissuearticles values(a,b);. On the other hand, Schiff bases 2a and 3a behaved as the most effective scavengers of the DPPH radical in methanol-water mixture insert ignore into journalissuearticles values(v:v, 1:3);. And, they were found to be have lower SC50 values in this mixture compared to pure methanol. In vitro cytotoxicity of these Schiff bases was studied against human cervical cancer cells insert ignore into journalissuearticles values(HeLa);, human breast adenocarcinoma cells insert ignore into journalissuearticles values(MCF-7);, and human normal embryonic kidney cells insert ignore into journalissuearticles values(HEK293);. For HeLa cell line, Schiff bases 1a-3a exhibited a litttle high activity than 1b, but very low activity than doxorubicin. Schiff bases 2b and 3b had no cytotoxicity against HeLa cell. For MCF-7 cell line, Schiff bases 1a, 3a, 1b and 3b nearly were inactive at 100 µM, whereas 2a increased cell proliferation in the all tested concentration range. Differently, Schiff base 2b showed the highest cytotoxicity and killed 90 percent of MCF-7 cells at concentration of 100 µM. For HEK-293, doxorubicin was strongly cytotoxic. Despite this, Schiff bases 1a, 3a and 3b were inactive, whereas the others showed little weak toxicity.
Keywords : Amino acid Schiff bases, Radical scavenging, Cytotoxicity, Doxorubicin