A HILIC stationary phase fuctionalized with glutathione by thiol-ene chemistry on monodisperse-porous polymer microparticles

Authors : Cigdem KİP

Pages : 21-31

Doi:10.15671/hjbc.567057

View : 23 | Download : 8

Publication Date : 2020-04-17

Article Type : Research Paper

Abstract :Monodisperse-porous microparticles functionalized a with a zwitterionic ligand were synthesized as a new stationary phase for hydrophilic interaction chromatography. Monodisperse-porous polyinsert ignore into journalissuearticles values(3-trimethoxysilylpropyl methacrylate-co-ethylene dimethacrylate);, polyinsert ignore into journalissuearticles values(TMSPM-co-EDMA); microspheres insert ignore into journalissuearticles values(6 μm in size); were obtained with different seed latex/monomer ratios and diluent compositions by multistage microsuspension copolymerization. The zwitterionic chromatographic ligand containing thiol moiety insert ignore into journalissuearticles values(i.e. glutathione); were covalently attached onto the TMSPM attached-polyinsert ignore into journalissuearticles values(TMSPM-co-EDMA); microparticles. The selected starting material allowed the direct attachment of zwitterionic ligand onto the support material using thiol-ene chemistry. The derivatized microparticles were slurry packed into the microbore columns with 2 mm i.d. and used as stationary phase for the separation of nucleosides in hydrophilic interaction chromatography with the plate numbers up to 54.000 plates/m. The results showed the usability  of  tailored poly insert ignore into journalissuearticles values(TMSPM-co-EDMA); microparticles as a stationary phase and thiol-ene chemistry in the manufacture of a chromatographic stationary phase with high efficiency in hydrophilic liquid chromatography applications.
Keywords : Thiol ene chemistry, Monodisperse porous microparticles, Hydrophilic interaction chromatography, nucleosides