Expression profiling and pathway analysis of iron oxide nanoparticles toxicity on human lung alveolar epithelial cell line using microarray analysis

Authors : Hasan TÜRKEZ, Mehmet Enes ARSLAN, Erdal SÖNMEZ, Abdulgani TATAR, Fatime GEYİKOĞLU, Metin AÇIKYILDIZ

Pages : 309-318

Doi:10.15671/hjbc.736803

View : 25 | Download : 1

Publication Date : 2020-07-06

Article Type : Research Paper

Abstract :Toxicogenomics is a developing area searching for cellular pathways and mechanisms including cancer, immunological diseases, environmental responses, gene-gene interactions and drug toxicity. Nanoparticles insert ignore into journalissuearticles values(NPs); become important candidates for analyzing in toxicogenomic experiments because of their unusual properties in various biological activities. Therefore, we examined the nanotoxicity of iron oxide insert ignore into journalissuearticles values(Fe2O3); on gene expression profiling of human alveolar epithelial cells insert ignore into journalissuearticles values(HPAEpiC); in the study. For this aim, iron oxide nanoparticles were synthesized by zone melting method and characterized via using X-ray crystallography insert ignore into journalissuearticles values(XRD); and transmission electron microscope insert ignore into journalissuearticles values(TEM); techniques. Cell viability and cytotoxicity were determined by 3-insert ignore into journalissuearticles values(4,5-dimethyl-thiazol-2-yl); 2,5-diphenyltetrazolium bromide insert ignore into journalissuearticles values(MTT);, neutral red insert ignore into journalissuearticles values(NR); and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); release tests. Whole-genome microarray expression analysis was performed to explore the effects of iron oxide nanoparticles on gene expression in cultured human alveolar epithelial cells. For further analyses, these genes were functionally classified by using DAVID insert ignore into journalissuearticles values(The Database for Annotation, Visualization and Integrated Discovery); with gene ontology insert ignore into journalissuearticles values(GO); analysis. The results from this study indicated that iron oxide-mediated toxicity directly or indirectly affecting the regulation of cell proliferation, response to hormone stimulus, estrogen stimulus, cytokine activity and blood circulation by stimulating diverse genes.
Keywords : Iron oxide nanoparticles, Microarray analysis, Toxicogenomics, Human alveolar epithelial cells, DAVID analysis