11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection

Authors : Burçak DEMİRBAKAN

Pages : 271-281

Doi:10.15671/hjbc.1240299

View : 63 | Download : 59

Publication Date : 2023-07-16

Article Type : Research Paper

Abstract :In this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate insert ignore into journalissuearticles values(ITO-PET); electrode modified with the 11-insert ignore into journalissuearticles values(triethoxysilyl); undecanal insert ignore into journalissuearticles values(11-TESU); agent was developed for the detection of tumour necrosis factor-alpha insert ignore into journalissuearticles values(TNF-α); in serum. The developed biosensor was observed with electrochemical impedance spectroscopy insert ignore into journalissuearticles values(EIS);, cyclic voltammetry insert ignore into journalissuearticles values(CV); techniques, square wave voltammetry insert ignore into journalissuearticles values(SWV); and single frequency impedance insert ignore into journalissuearticles values(SFI); technique which is utilized for the specific interaction between anti-TNF-α and TNF-α antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed insert ignore into journalissuearticles values(SEM);. All parameters such as 11-TESU concentration, anti-TNF-α concentration and anti-TNF-α incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-αelectrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection insert ignore into journalissuearticles values(from 0.03 pg mL-1 to 3 pg mL-1);. The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.
Keywords : İndiyum kalay oksit ITO, 11 trietoksisilil, undekanal 11 TESU, Tümör nekrozis faktör alfa