Examination of the composition and microorganisms in the oral microflora with to Immunological parameters identification of Saliva

Authors : Abdulrazaq Mohammad SABAH, Ikonnikova NATALIYA

Pages : 114-118

Doi:10.22399/ijcesen.1011764

View : 17 | Download : 12

Publication Date : 2021-11-30

Article Type : Research Paper

Abstract :The oral mucosa is a mucous membrane that connects the lips to the pharyngeal and gastrointestinal mucosa. Its particular properties enable it to function as a gatekeeper, regulating the impact of inhaled and ingested antigens, and the degrees of inflammation and immunological responses tolerated in a normal healthy mouth cavity. The objectives of this study are to: insert ignore into journalissuearticles values(1); quantification of the composition of the oral micro flora in individuals of 18-50 years old; insert ignore into journalissuearticles values(2); examination of the oral cavity microorganisms insert ignore into journalissuearticles values(culture-morphological and microscopic studies);; and insert ignore into journalissuearticles values(3); determination the biochemical parameters insert ignore into journalissuearticles values(lysozyme concentration, active acidity level); of the oral fluid insert ignore into journalissuearticles values(saliva);. The research evaluated 65 people between the ages of 18 and 50. Swabs from the oral cavity insert ignore into journalissuearticles values(within the cheeks); were used to inoculate agar media insert ignore into journalissuearticles values(MPA, Endo medium);. Colonies insert ignore into journalissuearticles values(CFU); on the agar medium`s surface were counted and converted to an area of the oral cavity insert ignore into journalissuearticles values(4 cm2);. The oral fluid was collected in the morning, on an empty stomach, before brushing the teeth, into a sterile test tube with a tight-fitting cover. This was done by thoroughly cleaning the individual`s mouth with a sterile 0.9% NaCl solution. We used indicator strips of paper with pH ranges of 5.4–7.8, 0.2 steps. Oral fluid centrifuged insert ignore into journalissuearticles values(2000 rpm for 20 min);. Indicator paper was dropped over the supernatant and the pH was calculated using a standard scale. Saliva from patients was collected in 1 ml polyethylene tubes, diluted 1:1 with saline, and centrifuged for 10 minutes at 1500 rpm. We studied the slurry. For this reason, the measurements are done in calibrated test tubes with 3 ml of the investigated lysozyme solution each time. The control is 0.5 M phosphate buffer. The research revealed that those with periodontitis and caries had pH alterations in their saliva. This group`s pH was 5.03. Compared to the healthy group, the pH decreased. The results showed that individuals with dental and periodontal issues had substantially lower levels of Lysozyme than healthy people. A severe decrease of nonspecific oral tissue resistance lowers oral fluid resistance to pathogens. Morover, Oral bacteria were all Amoxicillin resistant. Lactobacillus.
Keywords : Mucosa, Micro flora, Oral cavity