Growth and norharmane production of Chroococcus minutus under various stress conditions

Authors : Tunay Karan, Omer Kayir, Zekeriya Altuner, Ramazan Erenler

Pages : 10-15

Doi:10.32571/ijct.348526

View : 88 | Download : 8

Publication Date : 2018-03-05

Article Type : Research Paper

Abstract :Cyanobacterium samples were collected from fresh water of Tokat city in Turkey, and then isolation and cultivation of Chroococcus minutus were achieved successfully. TLC insert ignore into journalissuearticles values(Thin layer chromatography); and HPLC insert ignore into journalissuearticles values(High Performance Liquid Chromatography); analyses revealed that the C. minutus consisted of norharmane as a major product. So amount of norharmane was determined during the growth process. Growth and norharmane production of C. minutus were executed under salt stress and pH stress conditions. The most growth and the highest production of norharmane were detected at 16 th day. Therefore inoculation process was performed at 16 th day. Salt stress was evaluated at 0.5, 1.0, 3.0 and 5.0 M concentrations. The most norharmane was synthesized by C. minutus at 5 M concentration. The norharmane production and the growth were higher at pH 9 than that of the pH 5. Most norharmane was produced at pH 7.
Keywords : Chroococcus minutus, cyanobacteria, HPLC, norharmane

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