Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography

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International Journal of Chemistry and Technology
Volume:3 Issue:1
Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography

Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography

Authors : Hüseyin AKŞİT, Mehmet KEÇECİ, İbrahim DEMİRTAŞ, Nusret GENÇ

Pages : 72-76

Doi:10.32571/ijct.577183

View : 57 | Download : 8

Publication Date : 2019-06-28

Article Type : Research Paper

Abstract :In this study; it was aimed at the isolation of rosmarinic acid insert ignore into journalissuearticles values(RosA); from Ocimum basilicum insert ignore into journalissuearticles values(OB); with a selective extraction and rapid isolation technique using flash chromatography insert ignore into journalissuearticles values(FC);. For this purpose, dried leaves of OB were extracted with n-hexane, dichloromethane, and methanol, respectively. The methanol extract insert ignore into journalissuearticles values(ME); was extracted with dichloromethane and ethyl acetate using separation funnel by suspending with water. The ethyl acetate layer was dried with anhydrous Na 2 SO 4 and the solvent was evaporated to give a rosmarinic acid-rich extract. Then the ethyl acetate extract insert ignore into journalissuearticles values(EAE); was separated over silica gel column using FC, and thus RosA was isolated with high purity.
Keywords : Rosmarinic acid, flash chromatography, Ocimum basilicum

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