Cytotoxic, apoptotic, and necrotic effects of silver nanoparticles biosynthesized using Origanum majorana extract

Authors : Ramazan ERENLER, Esma Nur GEÇER, Büşra MORAN BOZER

Pages : 142-146

Doi:10.32571/ijct.1187754

View : 49 | Download : 9

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :application. Anticancer activity of Origanum majorana extract and silver nanoparticles was investigated by MTT assay using human pancreatic adenocarcinoma cell lines insert ignore into journalissuearticles values(Capan-1);, mouse normal fibroblast cell lines insert ignore into journalissuearticles values(L929);, and human colon adenocarcinoma cell line insert ignore into journalissuearticles values(Caco-2);. AgNPs@Om exhibited the excellent cytotoxic effect on Capan-1 cell lines with the cell viability of 29.17% and 25.70%, 22.49%, 21.16% at 1.0, 0.5, 0.25, 0.125 μg/mL respectively. However, the extract was determined to show moderate activity. A considerable fall in the viable cell number in Caco-2 cell lines was observed after the treatment of AgNPs@Om. The viable cells of Caco-2 cell lines were detected as 32.0% at 1.0 μg/mL with the treatment of nanoparticles. Yet, the viable Caco-2 cells were determined as 48.2% with the treatment of O. majorana extract. Both extract and nanoparticles had no impact on the viability of non-tumor cells insert ignore into journalissuearticles values(L929);. Moreover, nanoparticles induced apoptosis. Consequently, AgNPs@Om may be a promising anticancer drug candidate.
Keywords : Silver nanoparticles, natural products, Origanum majorana, anticancer activity

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