Antifungal activity and optimization procedure of microwave-synthesized silver nanoparticles using linden (Tilia rubra subsp. caucasica) flower extract

Authors : Uğur YİĞİT, Muharrem TÜRKKAN

Pages : 25-37

Doi:10.32571/ijct.1194356

View : 89 | Download : 111

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :The present study used linden [Tilia rubra DC. subsp. caucasica insert ignore into journalissuearticles values(Rupr.);] flower extract as a reducing and coating agent to create silver nanoparticles insert ignore into journalissuearticles values(AgNPs);. The Face-Centered Central Composite Design insert ignore into journalissuearticles values(FCCD); of Response Surface Methodology insert ignore into journalissuearticles values(RSM); was used to investigate the combined effect of four different synthesis variables in order to obtain the maximum amount of AgNPs produced. Optimal AgNP production was achieved within the investigated range when the AgNO3 concentration, plant extract amount, microwave power, and time were 10 mM, 2.5 ml, 800 watts, and 90 seconds, respectively. The Ultraviolet–Visible Spectroscopy insert ignore into journalissuearticles values(UV–Vis);, Fourier Transform Infrared Spectroscopy insert ignore into journalissuearticles values(FT–IR);, Scanning Electron Microscopy insert ignore into journalissuearticles values(SEM);–Energy Dispersive X-ray Spectroscopy insert ignore into journalissuearticles values(EDS);, and Transmission Electron Microscopy insert ignore into journalissuearticles values(TEM); were utilized to characterize the synthesized AgNPs. In addition, in vitro experiments revealed that the EC50, minimum inhibitory concentration insert ignore into journalissuearticles values(MIC);, and minimum fungicidal concentration insert ignore into journalissuearticles values(MFC); values of synthesized AgNPs for seven Phytophthora insert ignore into journalissuearticles values(P. cactorum, P. capsici, P. cinnamomi, P. citrophthora, P. megasperma, P. nicotianae, and P. palmivora); species varied between 46.38 and 119.36 µg ml-1, 225 and 450 µg ml-1, and 225 and 900 µg ml-1, respectively. The findings of this study suggest that AgNPs synthesized with linden flower extract should be investigated further for use in the treatment of Phytophthora spp.-caused diseases.
Keywords : Silver nanoparticles, Tilia rubra subsp caucasica, face centered central composite design, Phytophthora, toxicity

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