Morinda reticulata Gamble Tubers: A Potent Hepatoprotective Agent Against CCl4 and Paracetamol Induced Hepatotoxicity in Wistar Albino Rats

Authors : Raju ASİRVATHAM, Joshila AKHİL

Pages : 26-39

View : 24 | Download : 13

Publication Date : 2020-06-01

Article Type : Research Paper

Abstract :Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs. The hepatoprotective study was aimed to explore the extracts of Morinda reticulata Gamble for hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity on Wistar albino rats. Treatment group rats insert ignore into journalissuearticles values(4 groups, n=6 in each); were received either 200 mg/kg and 400 mg/kg of ethanol or aqueous extract of M. reticulata insert ignore into journalissuearticles values(p.o); and one group insert ignore into journalissuearticles values(n=6); was received 25 mg/kg of silymarin insert ignore into journalissuearticles values(p.o); twice daily for 7 days. CCl4 insert ignore into journalissuearticles values(1.5 ml/kg body weight, p.o); or paracetamol insert ignore into journalissuearticles values(2 g/kg); intoxication was done after the last dose of treatment and standard drug administration. Hepatoprotective potential was determined on the basis of measurement of hepatic serum marker enzymes such as Alanine Transaminase insert ignore into journalissuearticles values(AST);, Aspartate transaminase insert ignore into journalissuearticles values(AST);, Alkaline Phosphatase insert ignore into journalissuearticles values(ALP);, Total bilirubin and liver weight. Further, liver tissue was subjected to histopathological studies. HPTLC studies were done to determine the presence of phytochemical constituents. Pretreatment with silymarin, ethanol and aqueous tuber extract of M. reticulata significantly insert ignore into journalissuearticles values(p < 0.001); reduced the CCl4 or paracetamol induced elevated AST, ALT, ALP, Total bilirubin level in serum and also reduced liver weight and total protein levels. HPTLC study of ethanol extract showed six distinct peaks for chemical constituents. The present study indicated that ethanol and aqueous tuber extract of M. reticulata has potential hepatoprotective effect against chemicals and it can be used as a clinical remedy for hepatotoxicity disorders.
Keywords : Hepatoprotective, HPTLC fingerprint, Morinda reticulata Gamble, paracetamol, sylimarin