Neuroprotective Effects of Rutin and Quercetin Flavonoids in Glaucium corniculatum Methanol and Water Extracts

Authors : Fatma Gonca Koçancı, Belma Aslim

Pages : 85-93

Doi:10.21448/ijsm.363347

View : 11 | Download : 8

Publication Date : 2017-11-25

Article Type : Research Paper

Abstract :Neurodegenerative diseases insert ignore into journalissuearticles values(NDs); are characterized by loss of neurons. NDs are associated with development of inflammation. Existing drugs against NDs only delay the progression; however, they do not provide a cure. The studies for the treatment of NDs focused on to discover natural products that have the potential of anti-inhibition and anti-radical properties. The Papaveraceae family members are important for the synthesis of pharmaceutically compounds such as flavonoids which act like anti-inflammatory drugs. In this study, methanol and water extracts of Glaucium corniculatum, a member of the Papaveraceae family, were analysed for flavonoid compounds. The effects of extracts on neuronal PC12 cells viability was determined. The anti-inflammatory effects of extracts were assessed by measuring the levels of IL-6 and IL-10 cytokines on hydrogen peroxide insert ignore into journalissuearticles values(H2O2);-stimulated PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been found to be as major. The amount of Rutin was higher in methanol insert ignore into journalissuearticles values(45 μg/ml); than water insert ignore into journalissuearticles values(41 μg/ml);. Quercetin was better extracted with methanol insert ignore into journalissuearticles values(12 μg/ml); than water insert ignore into journalissuearticles values(10 μg/ml);. None of the tested extracts were cytotoxic even to PC12 cells. Both extracts showed an anti-inflammatory effect in a dose dependent manner. The water extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased 79 fold according to the H2O2 treated group and IL-10 secretion increased to 87 fold according to the control group. This study is an evidence that the Rutin and Quercetin flavonoids detected in G. corniculatum methanol and water extracts have a neuroprotective effect through anti-inflammation.
Keywords : Glaucium corniculatım, flavonoid, neurodegenerative diseases, anti inflammation