Enhancement of Plant Regeneration in Lemon Balm (Melissa officinalis L.) with Different Magnetic Field Applications

Authors : Canan ULGEN, Arzu YILDIRIM, Arzu TURKER

Pages : 99-108

Doi:10.21448/ijsm.677102

View : 21 | Download : 10

Publication Date : 2020-06-13

Article Type : Research Paper

Abstract :Melissa officinalis L. insert ignore into journalissuearticles values(lemon balm); is a valuable medicinal and aromatic plant in the Lamiaceae family. Two independent experiments were performed to improve the productivity of the plant regeneration. Firstly, the most efficient in vitro culture system of M. officinalis was determined using 7 different explant types insert ignore into journalissuearticles values(leaf, petiole, stem, root, axillary buds, shoot buds and cotyledon buds); on medium containing Murashige and Skoog minimal organics insert ignore into journalissuearticles values(MSMO); medium with different concentrations and combinations of plant growth regulators insert ignore into journalissuearticles values(PGRs);. Micropropagation was obtained only with explants containing meristematic cells insert ignore into journalissuearticles values(axillary buds, shoot tip buds and cotyledon buds);. Lemon balm had a very low regeneration capacity and in the second part of the experiment, enhancement of regeneration was aimed with the applications of different magnetic fields insert ignore into journalissuearticles values(MFs);. Two different MFs insert ignore into journalissuearticles values(50 and 100 mT); were generated using neodymium block magnets. There was no MF exposure with control treatment. Three different explants insert ignore into journalissuearticles values(axillary, shoot tip and cotyledon buds); were cultured on media including BA in combination with indole-3-acetic acid insert ignore into journalissuearticles values(IAA); or naphthalene acetic acid insert ignore into journalissuearticles values(NAA); with the application of two different MFs at 1 hour duration. As a result, it was determined that MF applications enhanced the regeneration capacity of M. officinalis and the best shoot formation was observed with axillary bud explant cultured in 1.5 mg/L BA at 100 mT MF application for 1 hour duration.
Keywords : In vitro culture, Lemon balm, Magnetic field, Melissa officinalis, Micropropagation