Alleviation Effects of Diosmetin on H2O2-Induced Oxidative Damage in Human Erythrocytes

Authors : Mucip GENİŞEL, Fatma YILDIZOĞLU

Pages : 31-39

Doi:10.21448/ijsm.793336

View : 20 | Download : 10

Publication Date : 2021-03-08

Article Type : Research Paper

Abstract :Free radicals insert ignore into journalissuearticles values(FRs); are formed in the high amounts result of the metabolic imbalance in cells and tissue. These radicals-induced oxidative damages constitute the basis of many diseases. Organisms have antioxidant defence systems insert ignore into journalissuearticles values(ADS); to eliminate the destructive effects of the oxidative damage. In addition to these antioxidant systems, dietary flavonoids have the antioxidant effect and the protective role against oxidative damage. In the present study, it was investigated whether a flavonoid derived diosmetin insert ignore into journalissuearticles values(10, 50, and 100 µM); have the elimination potential on hydrogen peroxide insert ignore into journalissuearticles values(H2O2);-induced oxidative damage in erythrocyte culture by using biomarkers such as lipid peroxidation insert ignore into journalissuearticles values(LP); level, catalase insert ignore into journalissuearticles values(CAT);, total superoxide dismutase insert ignore into journalissuearticles values(SOD); activity and changes of SOD isozymes containing the manganese SOD insert ignore into journalissuearticles values(Mn SOD); and the cupper-zinc SOD insert ignore into journalissuearticles values(CuZn SOD);. CAT, total SOD, Mn SOD and CuZn SOD activities showed a serious decline with H2O2 treatment, but diosmetin addition significantly increased their activities. While the H2O2 application critically increased LP products in erythrocytes, diosmetin considerably reduced these oxidative damage products. In conclusion, it has been determined that diosmetin can moderate oxidative damage in human erythrocytes by activating or protecting the ADS.
Keywords : Diosmetin, Flavonoid, Oxidative damage, Human erythrocyte