Antibacterial and Antioxidant Activity of Isoflavans from the Roots of Rhynchosia ferruginea and In Silico Study on DNA Gyrase and Human Peroxiredoxin

Authors : Kalid HUSSEİN, Rajalakshmanan ESWARAMOORTHY, Yadessa MELAKU, Milkyas ENDALE ANNİSA

Pages : 321-336

Doi:10.21448/ijsm.962120

View : 38 | Download : 13

Publication Date : 2021-12-26

Article Type : Research Paper

Abstract :Rhynchosia ferruginea insert ignore into journalissuearticles values(Udusalim, Afan Oromo); is a medicinal plant traditionally used to treat skin infection, intestinal problems and amoebiasis. Silica gel chromatographic separation of dichloromethane/methanol insert ignore into journalissuearticles values(1:1); roots extract yielded isoflavan insert ignore into journalissuearticles values(1);, isoflavene insert ignore into journalissuearticles values(2); and 1, 3-dilinoleoyl-2-stearoylglycerol insert ignore into journalissuearticles values(3);, reported herein for the first time from the genus. Antibacterial activity was examined using disc diffusion method against E. coli, S. aureus, P. aeruginosa and S. pyogenes. AutoDoc vina 4.2 soft ware was used for molecular docking analysis of compounds against human peroxidoxin 5 and DNA gyrase B enzymes. Isoflavan insert ignore into journalissuearticles values(1); displayed zone of inhibition of 9.67 ± 0.58 mm and 10.67 ± 0.58 mm whereas isoflavene insert ignore into journalissuearticles values(2); showed 10.33 ± 1.15 mm and 10 ± 1.00 mm against E. coli and S. aureus, respectively, compared to ciprofloxacin insert ignore into journalissuearticles values(15.67±0.58 mm for both strains);. DPPH radical scavenging assay of the dichloromethane/methanol insert ignore into journalissuearticles values(1:1); roots extract and isoflavan insert ignore into journalissuearticles values(1); exhibited better radical scavenging activity with IC50 value of 17.7 and 32, respectively. Molecular docking analysis revealed that 1 and 2 exhibited similar binding affinity of -7.4 kcal/mol compared to ciprofloxacin insert ignore into journalissuearticles values(-7.3 kcal/mol);. In silico analysis against human peroxidoxin 5 insert ignore into journalissuearticles values(PDB ID: 1HD2); revealed minimum binding affinity of - 3.7 and - 2.0 kcal/mol for compounds 1 and 2, respectively, compared to ascorbic acid insert ignore into journalissuearticles values(-4.9 kcal/mol);. The in vitro antibacterial and antioxidant activity of compounds 1 and 2 suggest the potential use of these compounds as drug lead candidates which corroborate with the traditional uses of the roots of R. ferruginea.
Keywords : Antibacterial activity, Rhynchosia ferruginea, Radical scavenging activity, Molecular docking

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