Assessment of genetic diversity based on agro-morphological traits and ISSR molecular markers in einkorn wheat (Triticum monococcum ssp. monococcum) landrace populations from Turkey

Authors : Suliman ZOMMİTA, Gülgez Gökçe YILDIZ, Nusret ZENCİRCİ, Özlem ÖZBEK, Ömer Can ÜNÜVAR, Ercan Selçuk ÜNLÜ

Pages : 86-105

View : 19 | Download : 11

Publication Date : 2023-03-26

Article Type : Research Paper

Abstract :The aim of this study is to investigate genetic diversity in 48 einkorn insert ignore into journalissuearticles values(Triticum monococcum L. ssp. monococcum); landraces grown in agricultural areas of Bolu and Kastamonu. Therefore, variation in seven agro-morphological traits was investigated. Agro-morphological traits such as leaf weight insert ignore into journalissuearticles values(mg);, coleoptile length insert ignore into journalissuearticles values(cm);, root number insert ignore into journalissuearticles values(n);, root length insert ignore into journalissuearticles values(cm);, fresh root weight insert ignore into journalissuearticles values(mg);, and dry root weight insert ignore into journalissuearticles values(mg); were examined by the coefficient of variation, ANOVA, and principal components analysis. The highest coefficient of variation insert ignore into journalissuearticles values(%); was observed in fresh root weight insert ignore into journalissuearticles values(FRW = 52.09%);, while the lowest was in leaf weight insert ignore into journalissuearticles values(LW = 8.9%);. Principal Component Analysis insert ignore into journalissuearticles values(PCA); was calculated as 76.93% variation in two main components. For molecular characterization, data obtained with iSSR primers were analyzed with the population genetics analysis program PopGene insert ignore into journalissuearticles values(ver. 1.32);. According to PopGene results, the mean number of alleles, the mean number of effective alleles, and average genetic diversity values were calculated as na = 2, nea = 1.33, and h = 0.13, respectively. Among the agro-morphological traits, germination power, root number, and coleoptile length appeared to be reliable traits. The results show that the use of morphological characters alone for genetic diversity in populations is not sufficient to determine the difference between populations and their genetic structure.
Keywords : Agro Morphological traits, Einkorn Wheat, Triticum monococcum ssp monococcum, iSSR, Wheat landraces