Antioxidant activity, analgesic activity, and phytochemical analysis of Ammi majus (L.) extracts

Authors : Otman ELGUOURRAMI, Soufiane DRIOUA, Mouna AMEGGOUZ, Najoua SALHI, Karima SAYAH, Gokhan ZENGİN, Ahmed ZAHIDI, Anass DOUKKALI, Hanane BENZEID

Pages : 23-37

View : 12 | Download : 9

Publication Date : 2023-03-26

Article Type : Research Paper

Abstract :Ammi majus insert ignore into journalissuearticles values(L.); is commonly used to cure many diseases in Moroccan folk medicine, especially vitiligo. This research tries to evaluate the phytochemical constituents of two aqueous extracts insert ignore into journalissuearticles values(E1; Maceration; 48 h); and insert ignore into journalissuearticles values(E2: Infusion; 1h); and three organic fractions insert ignore into journalissuearticles values(F1: Cyclohexane);, insert ignore into journalissuearticles values(F2: Ethyl acetate insert ignore into journalissuearticles values(EtOAc);); and insert ignore into journalissuearticles values(F3: Ethanolic insert ignore into journalissuearticles values(EtOH);); of A. majus insert ignore into journalissuearticles values(L.); seeds, as well as to study the antioxidant and analgesic activity of the species. Phytochemical analysis, antioxidant activity insert ignore into journalissuearticles values(DPPH, FRAP, ABTS, and TAC tests);, and analgesic activity insert ignore into journalissuearticles values(writhing and tail immersion were induced by Acetic acid tests); were analyzed according to the literature. A quantitative phytochemical study indicate that the E1 had the highest content of total polyphenols insert ignore into journalissuearticles values(26.95 ± 0.53 mg GAE/g extract); and flavonoids insert ignore into journalissuearticles values(37.92 ± 0.46 mg QE/g extract);, while F3 showed a promising flavonol content insert ignore into journalissuearticles values(24.26±0.08 mg QE/g extract);. Tannins were found to be high in F1 insert ignore into journalissuearticles values(59.27 ± 0.16 mg CE/g extract); and F2 insert ignore into journalissuearticles values(57.65 ± 1.18 mg CE/g extract);. Antioxidant results reveals that DPPH insert ignore into journalissuearticles values(IC50 = 179.68 ± 0.47 μg/mL); and FRAP insert ignore into journalissuearticles values(EC50 = 367.03 ± 0.12 μg/mL); show to E1 a high antioxidant activity. Regarding the analgesic activity of the different studied extracts, it was found that E1 has a high peripheral analgesic effect with 62.32 % and a high central analgesic potential throughout the experimentation at 500 mg/kg. Our studies demonstrated for the first time that A. majus seeds extracts have high antioxidant and analgesic activities through different analysis techniques.
Keywords : Ammi majus, Phytochemical analysis, Antioxidant, Analgesic