The effect of extraction methods on the yields, chemical composition and antifungal activity of sawdust Cedrus atlantica Manetti essential oils

Authors : Chaimaa BOUYAHİA, Maria BENBOUZİD, Souad El Hajjaji EL HAJJAJİ, Miloudia SLAOUİ, Fatiha BENTATA, Mustapha LABHİLİLİ, Abdelhakim BOUYAHYA

Pages : 297-312

Doi:10.21448/ijsm.1047122

View : 71 | Download : 80

Publication Date : 2023-06-16

Article Type : Research Paper

Abstract :The aim of this work is to determine the effect of the extraction method on the yield, the chemical composition, and the antifungal activity of cedarwood essential oils insert ignore into journalissuearticles values(EOs); from sawdust of Moroccan Cedrus atlantica insert ignore into journalissuearticles values(C. atlantica);. EOs were extracted by different methods: hydrodistillation, soxhlet, maceration, and ultrasound. The chemical composition was determined using gas chromatography mass spectrometry insert ignore into journalissuearticles values(GC-MS); analysis. The yields of EOs were 5.60%, 11.68%, 4.82% and 9.33% for hydrodistillation, soxhlet, maceration and ultrasound, respectively. GC-MS revealed a diversity of chemical compounds depending on extraction methods. Indeed, the main compound of EOs obtained by soxhlet, maceration, and ultrasound was copalic acid methyl ester by a rate of 28.41%, 20.24%, and 24.17%, respectively. However, -himachalene insert ignore into journalissuearticles values(21.32%); followed by -himachalene insert ignore into journalissuearticles values(9.40%);, β-Copaen-4α-ol insert ignore into journalissuearticles values(7.71%); and longifolene insert ignore into journalissuearticles values(6.74%); are the main compounds of EO extracted by hydrodistillation. The antifungal activity of cedarwood EO was tested in vitro on two pathogenic fungi: Fusarium culmorum insert ignore into journalissuearticles values(F. Culmorum); and Botrytis cineria insert ignore into journalissuearticles values(B. Cinerea);. The minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); was determined by successive dilutions of the stock solutions. The extracted EOs by soxhlet, maceration and ultrasound showed the important inhibitory effect against B. cinerea insert ignore into journalissuearticles values(MIC=1.25 mL/L);. However, F. culmorum showed resistance towards all tested EOs. The finding of this study showed clearly that the volatile composition of EOs can be variable according to extraction. methods. Moreover, antifungal effects are depending on chemical composition of EOs but also to tested staris.
Keywords : Cedrus atlantica, Essential oils, Fusarium culmorum, Botrytis cinerea, Antifungal activity