The antifungal effect of propolis extract against cotton wild disease (Verticillium dahliae Kleb.)

Authors : Melike Mutlu YILMAZ, Yeşim KARA, Oktay ERDOĞAN

Pages : 257-268

Doi:10.21448/ijsm.1249463

View : 51 | Download : 41

Publication Date : 2023-06-16

Article Type : Research Paper

Abstract :The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar insert ignore into journalissuearticles values(PDA); media containing its ethanol extract insert ignore into journalissuearticles values(PE); at various concentrations insert ignore into journalissuearticles values(0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppm/mL); was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating insert ignore into journalissuearticles values(PHCVd3 isolate); and defoliating insert ignore into journalissuearticles values(PHCVd47 isolate); pathotypes of V. dahliae, the varieties Giza 45 insert ignore into journalissuearticles values(resistant);, Carmen insert ignore into journalissuearticles values(tolerant);, and Acala SJ2 insert ignore into journalissuearticles values(susceptible); treated with PEE insert ignore into journalissuearticles values(1 ppm/mL); were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppm/mL dose resulted in 75.2% suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4%. The lowest disease severity index insert ignore into journalissuearticles values(DSI); values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.
Keywords : Propolis, Verticillium dahliae, Antifungal activity, Alternative control