Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation

Authors : Rajendrabhai VASAIT, Shital BHAMARE, Sayali JAMDHADE, Yogita SAVKAR

Pages : 175-189

Doi:10.21448/ijsm.1258717

View : 64 | Download : 65

Publication Date : 2023-06-16

Article Type : Research Paper

Abstract :This study was aimed at isolating potential antimicrobial compound insert ignore into journalissuearticles values(AMC); producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik insert ignore into journalissuearticles values(India);, and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B insert ignore into journalissuearticles values(28 mm);, followed by Staphylococcus aureus insert ignore into journalissuearticles values(26 mm);. Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B insert ignore into journalissuearticles values(18 mm);, followed by Staphylococcus aureus insert ignore into journalissuearticles values(16 mm);. A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μg/mL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.
Keywords : Antimicrobial compound, Bioactive compound, Antimicrobial activity, Bacillus amyloliquefaciens Bacillus siamensis