In vitro antioxidant activity and carbonic anhydrase inhibitory features of Ferula communis extracts

Authors : Fatma AYDIN, Zeynep Aleyna KAHRAMAN, Emir Alper TÜRKOĞLU, Müslüm KUZU, Zeki SEVEROĞLU

Pages : 592-598

Doi:10.31015/jaefs.2021.4.19

View : 24 | Download : 12

Publication Date : 2021-12-15

Article Type : Research Paper

Abstract :Carbonic anhydrases insert ignore into journalissuearticles values(CAs; EC 4.2.1.1); are essential family of metalloenzymes which catalyze the interconversion between carbon dioxide insert ignore into journalissuearticles values(CO2); and bicarbonate insert ignore into journalissuearticles values(HCO3-); in all organisms of three-domains of life. Huge amounts of attempts related to catalytic activity of CAs have been widely expanded to treat many clinical diseases. This study aimed to determine in-vitro antioxidant activities and human CA I insert ignore into journalissuearticles values(hCA I); and II insert ignore into journalissuearticles values(hCA II); inhibitory properties of Ferula communis extracts. Among all extracts of F. communis, the hexane extract has showed the best inhibitory profile on hCA I and II with IC50 values 8.68 µg/mL and 28 µg/mL and Ki values 2.026 µg/mL and 11.6 µg/mL, respectively. All extracts showed mild to moderate antioxidant activity. According to the results of DPPH assay, ethanol-water extract showed the highest activity with IC50: 0.1128±0.0066 value. Chloroform extract showed the highest activity on CUPRAC assay with the value of 1.305±0.037 mM Trolox equivalent/mg extract. However, further analytical, in-vivo and clinical studies are needed to confirm the activities of F. communis.
Keywords : Antioxidant activity, Carbonic anhydrase, Ferula communis, Inhibition