Plant Extract-Mediated Titanium Nanoparticles Using Nigella Sativa Extract and Titanium (IV) Dioxide Precursor: Cytotoxic Activity, Wound Healing Activity and Antibacterial Effect

Authors : Gülçin Uzlucan, Ayşegül İnam, Furkan Ozan Çöven, Tülay Öncü Öner
Pages : 404-414
Doi:10.7240/jeps.1746720
View : 83 | Download : 204
Publication Date : 2025-12-23
Article Type : Research Paper
Abstract :Nanoparticles synthesized through biological processes are utilized in cancer treatment, drug delivery systems, nanosensors, photoimaging, cosmetics, and medical applications. Extracts from Nigella sativa seeds have been traditionally used for centuries to treat numerous diseases. Current studies focus on the antidiabetic, anticancer, antibacterial, antifungal, anti-inflammatory, antiulcer, immune-enhancing, and hypoglycemic effects of these seeds. In this study, it was aimed to synthesize titanium nanoparticles by green synthesis method using N. sativa seeds and to investigate the effects of these nanoparticles on cytotoxicity, antibacterial, and wound healing activity. N. sativa seeds were extracted with distilled water and titanium nanoparticles were synthesized using titanium (IV) dioxide precursor. UV-VIS, SEM-EDX, FTIR, and XRD were used to characterize the nanoparticles. Then, the cytotoxic effects of the nanoparticles on HT-29 cells and wound healing effects on BJ and Caco-2 cells were investigated. Also, antibacterial activities were tested using Escherichia coli and Staphylococcus aureus. The cytotoxicity analysis demonstrated that cell viability decreased in a time- and dose-dependent manner. The lowest IC50 value was obtained at 72 hours with a value of 61.10±3.33 µg/mL. In the scratch assay, it was determined that 2.5 and 5 µg/mL titanium nanoparticle application showed no positive effect on wound healing. The MIC value for both bacteria was calculated as 0.125 mg/mL, and the inhibition zone diameters were measured as 10.81 mm against E. coli and 10.52 mm against S. aureus bacteria. This study has shown that titanium nanoparticles produced using N. sativa have potential for use in health and biomedical fields.
Keywords : Nigella sativa, TiO2-NP, sitotoksisite, yara iyileşme, antibakteriyel

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