Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD

Authors : Ceren EMİR, Nehir ÜNVER SOMER

Pages : 32-37

View : 41 | Download : 11

Publication Date : 2018-08-31

Article Type : Research Paper

Abstract :DOI: 10.5152/IstanbulJPharm.2018.416835 In this study, a reversed-phase, high pressure liquid chromatography insert ignore into journalissuearticles values(RP-HPLC); method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the  plant specimens was performed, using pre-packed columns with diatomaceous earth insert ignore into journalissuearticles values(Extrelut®);. The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid-water-acetonitrile insert ignore into journalissuearticles values(0.01:95:5); measured at a flow rate of 1 mL/min using a diode array detector insert ignore into journalissuearticles values(DAD);. The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.
Keywords : Galanthus alpinus Sosn var alpinus, Amaryllidaceae, lycorine, HPLC DAD

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