Ameliorative effects of melatonin on intestinal oxidative damage in streptozotocin-induced diabetic rats

Authors : Zatiye Ayça ÇEVİKELLİ YAKUT, Gizem Buse AKÇAY, Özge ÇEVİK, Göksel ŞENER

Pages : 160-167

View : 45 | Download : 14

Publication Date : 2020-12-30

Article Type : Research Paper

Abstract :investigated. Methods: Male Sprague-Dawley rats were assigned into 5 groups insert ignore into journalissuearticles values(10 rats in each);: Control, diabetes, diabetes+insulin, diabetes+melatonin, and diabetes+insulin+melatonin groups. Streptozotocin insert ignore into journalissuearticles values(60 mg/kg); was administered intraperitoneally to the rats to induce diabetes. At the end of 8 weeks of treatment, after blood glucose measurement and subsequent decapitation, glutathione insert ignore into journalissuearticles values(GSH); and malondialdehyde insert ignore into journalissuearticles values(MDA); levels and caspase-3, myeloperoxidase insert ignore into journalissuearticles values(MPO);, and superoxide dismutase insert ignore into journalissuearticles values(SOD); activities in the intestinal tissue were investigated. Results: In diabetic animals, elevated blood glucose levels caused oxidant damage in the intestinal tissue that was demonstrated with increased MDA levels, caspase and MPO activities, and decreased GSH levels and SOD activities. Although melatonin demonstrated more significant results than insulin, separate administration of both melatonin and insulin improved the oxidative damage parameters compared to the diabetes group. In the combined treatment group, all parameters were back to control levels statistically more significant when compared with the treatment-alone. Conclusion: Melatonin has been shown to protect intestinal tissue from diabetic oxidant damage. With insulin treatment in type I diabetes, melatonin supplements may increase the quality of life through reducing complications.
Keywords : Diabetes mellitus, oxidative damage, intestine, insulin, melatonin

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