Interaction of curcumin on cisplatin cytotoxicity in HeLa and HepG2 carcinoma cells

Authors : Merve BECİT, Sevtap AYDIN DİLSİZ, Nurşen BAŞARAN

Pages : 202-210

View : 70 | Download : 17

Publication Date : 2020-12-30

Article Type : Research Paper

Abstract :Background and Aims: Our study aimed to evaluate how curcumin affect cisplatin cytotoxicity in human cervical carcinoma insert ignore into journalissuearticles values(HeLa);, human hepatocellular carcinoma insert ignore into journalissuearticles values(HepG2);, and Chinese hamster lung fibroblast insert ignore into journalissuearticles values(V79); cells. Methods: The cytotoxicity was evaluated by MTT assay. Results: The IC50 values of curcumin were 404 μM and 320 μM in HeLa cells; 236 μM and 98.3 μM in HepG2 cells; 877 μM and 119 μM in V79 cells; for 24 h and 48 h, respectively. The IC50 values of cisplatin were 22.4 μM and 12.3 μM in HeLa cells; 25.5 μM and 7.7 μM in HepG2 cells; 15.4 μM and 4.9 μM in V79 cells; for 24 h and 48 h, respectively. Curcumin significantly decreased cisplatin cytotoxicity at 500 μM in HeLa cells and above 250 μM and 125 μM in HepG2 cells, for 24 h and 48 h, respectively. In V79 cells, curcumin significantly decreased the IC50 values of cisplatin above 500 μM and 125 μM for 24 h and 48 h. Conclusion: The results might contribute to the anticancer effect of the curcumin-cisplatin combination in cervical and hepatocellular carcinoma, but in order to support this result and determine its interactions with antineoplastic drugs, further studies are needed.
Keywords : Curcumin, cisplatin, cytotoxicity, HeLa cells, HepG2 cells

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