Investigation of aloe-emodin and Aloe vera gel extract on apoptosis dependent pathways in leukemia and lymphoma cell lines

Authors : Serap ERDEM KURUCA, Eda ÇANDÖKEN, Nuriye AKEV

Pages : 42-48

View : 53 | Download : 12

Publication Date : 2020-04-01

Article Type : Research Paper

Abstract :Background: The present study was designed to evaluate the mechanism of cytotoxic effects of AE relative to Aloe vera gel extract insert ignore into journalissuearticles values(AVG); on chronic myelogenous leukemia K562 and Burkitt’s lymphoma P3HR-1 cell lines. Methods: Cytotoxicity tests were assessed by tetrazolium bromide insert ignore into journalissuearticles values(MTT); assay. Annexin V-FITC/PI labelling was used for apoptosis/necrosis evaluation and western blotting for apoptotic molecules measurement. Results: It was shown that, AE has cytotoxic activity againts K562 and P3HR-1 cells with an IC50 value of 60.9 µM and 28 µM, respectively. AVG was found cytotoxic on K562 cells with an IC50 value of 243.2 µg/ml and ineffective on P3HR-1 cells. The ratio of apoptotic cells insert ignore into journalissuearticles values(46.7%); was high in only K562 cells after AVG treatment. The percentage of apoptotic cells insert ignore into journalissuearticles values(K562=34.1, P3HR-1=38.8); was higher than necrotic cells insert ignore into journalissuearticles values(K562=11.9, P3HR-1=16.6); after AE treatment. The main apoptosis pathway in both cell lines was found to be through caspase-3 and caspase-9 activations after AE treatment, caspase-8 was also activated in K562 cells but suppressed in P3HR-1 cells. Likewise caspase-9 was activated in K562 cells after AVG treatment. Conclusion: These results suggest that AE and AVG realized cell death activating apoptotic mechanisms in K562 and P3HR1cells through extrinsic and intrinsic pathways.
Keywords : Aloe vera, aloe emodin, cytotoxicity, leukemia, lymphoma, apoptosis, caspases

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